Construction of underexpression HSP90alpha and overexpression HSP90beta human hepatoma cell line HepG2 / 中国应用生理学杂志

<p><b>AIM</b>To construct underexpression HSP90alpha and overexpression HSP90beta human hepatoma cell line HepG2.</p><p><b>METHODS</b>The combined plasimid pSilencerHSP90alpha and pSmycHSP90beta were introduced into HepG2 by electroporation, respectively. The result of transfection was identified by Western-blotting and the curve of cell growth was drew by MTT. Observe the cell vitality and expression of HSP90.</p><p><b>RESULTS</b>Expression of HSP90 in transfected cell line was shown by Western-blotting: Compared with control, expression of HSP90 in the cells transfected with pSilencerHSP90alpha decreased, whereas that in the cells transfected with pSmycHSP90beta increased.The growth curves of the two groups of transfected cells was as the same as that of the control group.</p><p><b>CONCLUSION</b>The stable overexpression HSP90beta and underexpression HSP90alpha HepG2 cell lines were established.</p>

Phospholipase D and Pathogenic Microorganisms Invasion / 微生物学通报

Phospholipase D(PLD) is ubiquitous in bacteria,fungi,and mammal.In pathogenic microorganisms,PLD can be pathogenic determinant and play a role in spore generation.In mammalian cells,PLD functions in several signal transduction pathways,such as membrane transportation,mitosis regulation,and actin cytoskeleton regulation.In the process of pathogens invasion host cells,both of the pathogen and host cells’ PLD will be activated and a series of cascade reaction will be generated.During this process,pathogen’s PLD can regulate the polymerization and reorganization of its own actin filaments and induce the polymerization or reorganization of the host cell actin filaments near the foci,thus to promote the phagocytosis of the pathogen by host cell.Investigating the role of PLD activation in the infection will be significance for further understanding the molecular mechanism of pathogen-host cell interaction.